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trypan blue exclusion staining  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trypan blue exclusion staining
    Trypan Blue Exclusion Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypan+blue+exclusion+staining/Trypan+Blue/bio_rxiv__64898__2026__03__18__712644-166-10-14
    Average 99 stars, based on 1 article reviews
    trypan blue exclusion staining - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Alisol F 24-acetate attenuated metabolic dysfunction-associated steatohepatitis by targeting the KEAP1/NRF2-mediated macrophage pyroptosis.
    Article Snippet: To induce differentiation into bone marrow-derived macrophages (BMDMs), the cultures were treated with 20 ng/ml macrophage colony-stimulating factor (M-CSF; BioLegend, San Diego, CA, USA, Cat# 576404) for 7 days. .. Prior to any experimental stimulation, cell viability was assessed via trypan blue exclusion staining (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells.
    Article Snippet: Objective: Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition.. However, its large size hampers absorption and distribution of biomaterials.. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.

    Article Title: T6SS mutants exploit itaconate to support infection of phagocytes
    Article Snippet: Cells were subsequently washed with PBS, detached using TrypLE Express (Life Technologies), serially diluted, and plated on LB agar for bacterial enumeration at 1.5 or 3h post infection. .. Cell viability was assessed using trypan blue exclusion staining (Life Technologies). ..

    Article Title: DNA-Dependent Protein Kinase Inhibitors PI-103 and Samotolisib Augment CRISPR/Cas9 Knockin Efficiency in Human T Cells
    Article Snippet: .. At the end of the incubation, the cells were washed, counted with trypan blue exclusion staining (Life Technologies) and seeded in a G-Rex 24 well plate (Wilson Wolf). ..

    Article Title: Single-Cell Transcriptomics Reveals a Multi-Compartmental Cellular Cascade Underlying Elahere-Induced Ocular Toxicity in Rats.
    Article Snippet: Single-cell suspensions were prepared via enzymatic digestion (dispase II, 2.4 U/mL; collagenase A, 1 mg/mL; Roche) and gentle mechanical dissociation in Hank’s Balanced Salt Solution at 37 ◦C for 30–60 min. Tissues were triturated with wide-bore pipette tips to release individual cells, and the suspensions were filtered through 40 μm cell strainers to remove debris. .. Cell viability was assessed using trypan blue exclusion staining, and cells were counted on an automated cell counter (Countess II, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: DNA-dependent protein kinase inhibitors PI-103 and samotolisib augment CRISPR/Cas9 knock-in efficiency in human T cells.
    Article Snippet: .. At the end of the incubation, the cells were washed, counted with trypan blue exclusion staining (Life Technologies, Thermo Fisher Scientific, Waltham, Massachusetts, United States) and seeded in a G-Rex 24 well plate (Wilson Wolf). ..

    Article Title: Inhibition of NEK2 Promotes Chemosensitivity and Reduces KSHV-positive Primary Effusion Lymphoma Burden
    Article Snippet: .. Cells were then harvested, resuspended in PBS, and percent dead cells, as well as live cells per mL, were quantified using Trypan blue exclusion staining and a Countess II FL automated cell counter (Life Technologies). .. For measuring cell viability over time, PEL cells or PBMCs were seeded at a density of 1 × 10 4 cells/well or 1 × 10 5 cells/well, respectively, in triplicate in white 96-well plates (Greiner, 655083) in 100 μL volume.

    Article Title: A live-cell autophagy reporter reveals reversible vacuolation in naked mole-rat skin fibroblasts under lysosomal stress
    Article Snippet: .. Cell viability and density were determined using a haemocytometer and trypan blue exclusion staining (Gibco), and the cells were seeded (2 × 106 cells in 10 mL fresh media, T-75 flask). ..

    Cell Culture:

    Article Title: Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells.
    Article Snippet: Objective: Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition.. However, its large size hampers absorption and distribution of biomaterials.. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.

    Incubation:

    Article Title: DNA-Dependent Protein Kinase Inhibitors PI-103 and Samotolisib Augment CRISPR/Cas9 Knockin Efficiency in Human T Cells
    Article Snippet: .. At the end of the incubation, the cells were washed, counted with trypan blue exclusion staining (Life Technologies) and seeded in a G-Rex 24 well plate (Wilson Wolf). ..

    Article Title: DNA-dependent protein kinase inhibitors PI-103 and samotolisib augment CRISPR/Cas9 knock-in efficiency in human T cells.
    Article Snippet: .. At the end of the incubation, the cells were washed, counted with trypan blue exclusion staining (Life Technologies, Thermo Fisher Scientific, Waltham, Massachusetts, United States) and seeded in a G-Rex 24 well plate (Wilson Wolf). ..



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